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Mouse protocols
Standard protocol
Four 6-8-week-old female mice are immunised according to a 81-day protocol. This phase includes animal housing, subcutaneous and intraperitoneal injections as well as test bleeds according to the provisional calendar we provide you with at the beginning of the project.
D0: first
injection
Injection
Injection
Injection
Injection
Injection
Injection
D0: pre-immune bleed
Test bleed
Test bleed
D81: final bleed
Multisite® protocol
On request, we can perform our exclusive and proprietary Multisite® protocol which includes multiple additional injections as well as one additional test bleed as compared to the standard protocol. The protocol duration is increased from 81 to 94 days, but the chances of generating positive hybridomas is also dramatically increased.
D0: first
injection
Multiple injections
Injection
Injection
Injection
Injection
Injection
D0: pre-immune bleed
Test bleed
Test bleed
Test bleed
D94: final bleed
Rat protocol
Rat monoclonal antibody development projects involve the immunisation of two 8-week-old female animals. However, additional animals can be included in the protocol upon request.
D0: first
injection
Injection
Injection
Injection
Injection
D0: pre-immune bleed
Test bleed
D74: final bleed
These timelines are given as an indication. Depending on the sera immunoreactivity, modifications can be applied to the provisional calendar, including additional injections and/or test bleeds. Thanks to our experience, we bring you advice at any time to make the right decisions.