RECOMBINANT ANTIBODY

Covalab provides bio production services for antibodies (including their fragments). We are using eucaryotic systes and offering a large range of bioproduction scale starting from mililiter and up to 12 L bioreactor. All antibodies production occurs in our ISO-certified manufacturing facility.
Reactivity, high quality production and tailor made services is our main objective. Thanks to nearly 30 years of experience and our highly trained scientific team we can adapt our process to answer all your specific needs and meet your goal.

Tailor made service

Tailor-made service

Fee service

Fee for service

Animal free

Animal free

Large scale application

Large scale application

(from mg to g)

Eukaryotic production (CHO)

STEP 1Recombinant protein feasibility study

Bibliography study
Sequence analysis
Expression and purification strategy proposal (tag selection)
GO / NO GO step

STEP 2Development of eukaryotic expression model

Primers design
Gene synthesis including a tag (codon optimization)
Subcloning into an expression vector for eukaryotic model
Amplification and purification of the plasmid
Control by sequencing
GO / NO GO step

STEP 3Recombinant antibody pilot production in eukaryotic model

Transient transfection in CHO cells
Pilot production small scale volume (≈ 100 mL)
Antibody purification by affinity chromatography
Antibody characterization (SDS-Page, ELISA, flow cytometry, HPSEC, WB...) and yield estimation
Material transfert for validation
GO / NO GO step

STEP 4Recombinant antibody production in eukaryotic model

Transient transfection / Stable transfection in CHO cells
In vitro production (1 - 12 L)
Antibody purification by affinity chromatography
Polishing step (SEC, ion exchange...)
Antibody characterization (SDS-Page, ELISA, flow cytometry, HPSEC, WB...) and yield estimation (BCA...)
Endotoxin level, HCPs
Production, purification and quality control report