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Methylated arginine antibody (7E6)
clone : 7E6
As low as
€278.30
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00012708
Methylated arginine antibody (7E6)
| ID Covalab | mab0002-P |
|---|---|
| Product type | Primary antibodies |
| Clonality | Monoclonal antibody |
| Clone | (7E6) |
| Isotype | IgG1 / Kappa |
| Raised in | Mouse |
| Immunogen | Modified methyl arginine |
| Activity cross reaction | Reacts with all structure of methylated arginine including free and bound. Cross reacts with free and bound NG - Monomethyl Arginine. No cross reaction with free or bound Arginine. Ability to fix complement : unknown |
| Species | All Sp. |
| Labelling | None |
| Form | Purified (protein A) |
| Preservative | None |
| Storage instructions | Lyophilized powder stable for a minimum of 2 years at -20°C. Store reconstituted antibodies at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt. |
| Target | methylated arginine monomethyl arginine (MMA), asymetric dimethyl arginine (ADMA) and symetric dimethyl arginine (SDMA) |
| Applications | ChIP, ELISA, IP, WB |
| Working dilutions | Optimal dilution should be determined by the end user. The following are guidelines only : - ChIP : 1/100 to 1/10 000 - WB : 1/500 to 1/5 000 |
| Publication | * SR Green. Regulation of Citric Acid Cycle Enzymes and Related Pathways in the Skeletal Muscle of Hibernating Richardson’s Ground Squirrels, Urocitellus Richardsonii. Thesis Nov 2021 (WB) * SR Green et al. BBA - Proteins and Proteomics. 2020 May 20 * CL Childers et al. J Comp Physiol B. 2019 Jan 10 * Mattice JJL. Regulation of glutathione-based antioxidant defenses in response to dehydration stress in the African clawed frog, Xenopus laevis. Thesis 2018 * Alban C et al. Uncovering the protein lysine and arginine methylation network in Arabidopsis chloroplasts. PLoS One. 2014 Apr 18 :9(4):e95512. WB, plants * Abboud J and Storey KB. Novel control of lactate dehydrogenase from the freeze tolerant wood frog: role of posttranslational modifications. PeerJ 2013 1:e12: WB * Duong F. et al. Hepatitis C virus inhibits interferon signaling through up-regulation of protein phosphatase 2A. Gastroenterology. 2004 Jan:126(1):263-77. * Ong S.E. et al. Identifying and quantifying in vivo methylation sites by heavy methyl SILAC. Nat Methods. 2004 Nov:1(2):119-26. * Mowen K.A. et al. Arginine Methylation of STAT1 modulates IFNalpha/beta-induced transcription. Cell. (2004), Nov 24, 119(5),587-590. * Fabbrizio E. et al. Identifying and quantifying in vivo methylation sites by heavy methyl SILAC. Nat Methods. 2004 Nov:1(2):119-26. * Li H. et al. Lipopolysaccharide-induced methylation of HuR, an mRNA-stabilizing protein, by CARM1. Coactivator-associated arginine methyltransferase. J Biol Chem. 2002 Nov 22:277(47):44623-30. * Rho J. and al. The arginine-1493 residue in QRRGRTGR1493G motif IV of the hepatitis C virus NS3 helicase domain is essential for NS3 protein methylation by the protein arginine methyltransferase 1. J Virol. 2001 Sep:75(17):8031-44. * Zhu W. et al. Arginine methylation of STAT1 regulates its dephosphorylation by T cell protein tyrosine phosphatase. J Biol Chem. 2002 Sep 27:277(39):35787-90. |
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