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His-Tag antibody (HIS.H8 / EH158)
poly-histidine, polyhistidine
Histidine tagged proteins
6 His epitope tag, HHHHHH epitope tag,
Hexa His tag, 6X His tag
Histidine tagged proteins
6 His epitope tag, HHHHHH epitope tag,
Hexa His tag, 6X His tag
As low as
€275.90
In stock
Ref
00012927
His-Tag antibody (HIS.H8 / EH158)
| ID Covalab | mab90001-P |
|---|---|
| Product type | Primary antibodies, Tags |
| Clonality | Monoclonal antibody |
| Clone | HIS.H8 / EH158 |
| Isotype | IgG2b |
| Raised in | Mouse |
| Immunogen | HHHHHH (6x His) synthetic peptide conjugated to KLH |
| Activity cross reaction | Reacts with the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags) |
| Labelling | None |
| Form | Purified |
| Preservative | NaN3 0.05% |
| Storage instructions | Store at 4°C for 1 month. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt. |
| Target | poly-histidine |
| Applications | ELISA, ICC, IP, WB |
| Working dilutions | Optimal dilution should be determined by the end user. The following are guidelines only : WB 1:1000 - 1:3000 ICC 1:500 - 1:2000 |
| Publication | * Ossareh-Nazari B et al. Ubiquitylation by the Ltn1 E3 ligase protects 60S ribosomes from starvation-induced selective autophagy. J Cell Biol. 2014 Mar 17,204(6):909-17. |
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