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Aequorin, from the jellyfish Aequoria victoria, catalyses the oxidation of coelenterazine, but only in the presence of Ca2+ ions.
| ID Covalab | pab0005 |
|---|---|
| Product type | Primary antibodies |
| Clonality | Polyclonal antibody |
| Raised in | Rabbit |
| Immunogen | Purified Aequorin protein. |
| Activity cross reaction | Reacts with 22 kDa Aequorin protein from Jeffyfish. |
| Species | Jfi |
| Labelling | None |
| Form | Antiserum |
| Preservative | None |
| Storage instructions | Lyophilized powder stable for a minimum of 2 years at -20°C. Store reconstituted antibodies at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt. |
| Target | Aequorin |
| Applications | ELISA, WB |
| Working dilutions | Optimal dilution should be determined by the end user. The following are guidelines only : - WB : 1/1 000 to 1/10 000 |
| Publication | -Radford J.C. et al. Systematic G-protein-coupled Receptor Analysis in Drosophila melanogaster Identifies a Leucokinin Receptor with Novel Roles.J Biol Chem. 2002 Oct 11;277(41):38810-7. Epub 2002 Aug 5. - Brandenburger Y. et al. Measurement of perimitochondrial Ca2+ concentration in bovine adrenal glomerulosa cells with aequorin targeted to the outer mitochondrial membrane. Biochem J. 1999 Aug 1;341 ( Pt 3):745-53. |
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