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Specific Tissue Transglutaminase (tTG/TGase2) Colorimetric Microassay Kit : TG2-CovTest
Protein-glutamine gamma-glutamyltransferase 2
Tissue transglutaminase
Transglutaminase C (TG(C)), TGC, TGase C
Transglutaminase H, TGase H
Gene name: TGM2
Tissue transglutaminase
Transglutaminase C (TG(C)), TGC, TGase C
Transglutaminase H, TGase H
Gene name: TGM2
As low as
€488.80
In stock
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00014867
A colorimetric microassay for quantification of Tissue Tranglutaminase activity
| ID Covalab | opr0030 |
|---|---|
| Principe | The TG2-Covtest uses biotinylated T26 peptide (Biotin-pepT26) as the first substrate (amine-acceptor/acyl-donor) and an amine-donor/acyl-acceptor as a second substrate. In the presence of Tgase-2 (suspected to be contained in the samples), the γ carboxamide of the glutaminyl residue of the biotin-pepT26 is incorporated into the amine substrate to form biotinylated isopetide bound. The quantity of biotin incorporated is related to the Tgase-2 activity and is revealed by a peroxidase colorimetric reaction. |
| Applications | FA |
| Detection method | Colorimetric |
| Labelling | HRP |
| Activity cross reaction | Measurement of Transglutaminase-2 activity in biological samples with low or no interference with other TGs isoforms. |
| Species | All Sp. |
| Sample type | Biological fluids, cell and tissus lysates, recombinant Tgase-2 |
| Assay range | 2.5 - 40 µU/ml |
| Assay type | Colorimetric Microassay |
| Assay duration | 45 min |
| Storage instructions | DTT, SAv-HRP enzyme and opr0036 at -20°C. Other reagents at +4°C. |
| Target | Transglutaminase-2, TGase-2 |
| Uniprot ID | P21980 |
| Publication | * Barathi VA et al. Biomolecules 2023 Jun 13(7):1045 * Ulukan B et al. ACS Omega 2020 Oct. 20 * Miele AE et al. Sci Rep. 2019 Jul 2:9(1):9568 * Meshram DD et al. Eur J Pharmacol. 2017.09.035 * Carbone C et al. Amino Acids. 2016 Oct 19. * Luo R et al. J Am Heart Assoc. 2016 Jun 30,5(7). * Király R et al. Amino Acids. 2016 Jan,48(1):31-40. * Liu C et al. Hypertension. 2014 Feb,63(2):353-61. * Kumar A et al. BMC Cancer. 2014 Apr 11;14:256. * Caffarel MM et al. J Pathol. 2013 Oct,231(2):168-79. * Wang Z and Griffin M. PLoS One. 2013,8(3):e57017. * Perez Alea M et al. Amino Acids. 2013 Jan,44(1):245-50. * Hong GU et al. Cell Signal. 2013 Jun,25(6):1514-25. * Perez Alea M et al. Anal Biochem. 2009 Jun 15,389(2):150-6. * Meshram Dipak D et al. Curr. Issues Mol. Biol. 2024 May, 46:4251-70 |
| Product type | Kits |
| Sensitivity | 1.25 µU/ml |
| Description | Microtiter strips coated with amine substrate DTT EDTA (Negative Control) Reaction Buffer (Biotin-pepT26/CaCl2) Enzyme Tracer (SAv-HRP) Wash Buffer 10X Diluent Buffer 10X HRP Substrate Blocking Reagent Positive control. |
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