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Specific Microbial /Bacterial Transglutaminase (MTG) Colorimetric Microassay Kit : MTG-CovTest
microbial transglutaminase, MTg, Tg Bact
As low as
€497.30
In stock
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00029002
A colorimetric microassay for quantification of Bacterial Transglutaminase activity
| ID Covalab | opr0053 |
|---|---|
| Principe | The MTG-CovTest uses biotinylated peptide originated from the F-helix of myoglobin (Biotin-pepM) as the first substrate (amine-acceptor/acyl-donor) aand an amine-donor/acyl-acceptor as a second substrate. In the presence of MTgase (suspected to be contained in the samples), the gamma carboxamide of the glutaminyl residue of the Biotin-pepM is incorporated into the amine substrate to form biotinylated isopetide bound. The quantity of biotin incorporated is related to the transglutaminase activity and is revealed by a peroxidase colorimetric reaction. |
| Applications | FA |
| Detection method | Colorimetric |
| Labelling | HRP |
| Activity cross reaction | Measurement of Bacterial transglutaminase (MTGase) with low or no interference with other TGs isoforms. |
| Species | Str. |
| Assay type | Colorimetric Microassay |
| Assay duration | 45 min |
| Storage instructions | Store SAv-HRP enzyme at -20°C, other reagents at +4°C. |
| Target | Bacterial transglutaminase |
| Publication | * Veluchamy and al. Molecular Basis of Transglutaminase-2 and Muscarinic Cholinergic Receptors in Experimental Myopia: A Target for Myopia Treatment . MDPI, (Jun 2023). |
| Product type | Kits |
| Description | Microtiter strips coated with amine substrate Reaction Buffer ( Biotin-pepM ) Wash Buffer 10X Diluent Buffer 10X Enzyme Tracer (SAv-HRP) Enzyme Tracer Diluent buffer 10X HRP Substrate Blocking Reagent |
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