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Nucleolin pT76 antibody
Protein C23
Gene name : NCL
phospho Nucleolin, Nucleolin phospho-Thr 76, NCL-T76p
Gene name : NCL
phospho Nucleolin, Nucleolin phospho-Thr 76, NCL-T76p
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00106854
Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
| ID Covalab | pab0970-P |
|---|---|
| Product type | Primary antibodies |
| Clonality | Polyclonal antibody |
| Raised in | Rabbit |
| Immunogen | Synthetic peptide of human Nucleolin with a phoshothreonine at position 76 |
| Activity cross reaction | Reacts with Nucleolin with phoshothreonine 76 (pT76) from human and mouse. Do not cross react with phoshothreonine 84, 92 & 106 (pT76 and pT92). The detection of this P-NCL can be done during mitosis or in the centrosome during G1 phase, no signal in G2 phase. |
| Species | Hu, Ms |
| Labelling | None |
| Form | Purified (protein A) |
| Preservative | None |
| Storage instructions | Short term storage at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt. |
| Target | Nucleolin pT76 |
| Uniprot ID | P19338 |
| Gene ID | 4691 |
| Applications | IF, IP, WB |
| Working dilutions | Optimal dilutions should be determined by the end user. The following are guidelines only: WB 1:100 - 1:1000 IF 1:50 - 1:300 |
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