GAPDH antibody (GA1R)

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
Peptidyl-cysteine S-nitrosylase GAPDH
Gene name : GAPDH, GAPD
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00012923
As GAPDH is an enzyme expressed stably and at high level in a wide range of cells and tissues, it is commonly used as loading control
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ID Covalab mab90009-P
Product type Loading controls, Primary antibodies
Clonality Monoclonal antibody
Clone GA1R
Isotype IgG1
Raised in Mouse
Immunogen Recombinant GAPDH
Activity cross reaction Reacts with native and denatured forms of GAPDH (∼37kDa). Cross react with GAPDH from E. Coli (BL-21) bacteria, Sf9 insect, Saccharomyces cerevisiae (yeast), human, mouse, rat, rabbit, chicken, and hamster. GAPDH from other species may also be detectable.
Species E. coli, Hu, Ms, Oth, Rat
Labelling None
Form Purified
Preservative NaN3 0.05%
Storage instructions Short term storage at +4°C (add 0.1% NaN3).
For extended periods store in aliquots at -20°C.
Antibodies are guaranteed for 6 month from date of receipt.
Target GAPDH
Uniprot ID P04406
Gene ID 2597
Applications ELISA, FC, IF, IHC - P, WB
Working dilutions Optimal dilution should be determined by the end user.
The following are guidelines only :
WB (1:1000 - 1:10000)
FC (2 µg/ml)
Publication * Bellamri N. et al., Effects of Ruxolitinib on fibrosis in preclinical models of systemic sclerosis,International Immunopharmacology ,2023 Jan
* Basson A et al., The Streptococcus agalactiae R3 surface protein is encoded by sar5, PLoS ONE,2022 Jul 29:17(7):e0263199
* A Justet et al. FGF19 is Downregulated in Idiopathic Pulmonary Fibrosis and Inhibits Lung Fibrosis in Mice, Am J Respir Cell Mol Biol. 2022 May 12
* Dragset MS et al. The Streptococcus agalactiae R3 surface protein is encoded by sar5, bioRxiv, 2022 Jan 17.
* Menou A et al. FASEB J. 2017 Nov 9. pii: fj.201700583R.
* Roussilhon C et al. Sci Rep. 2017 Feb 9;7:42243. (WB, Ms)
* Veréb Z et al. Sci Rep. 2016 May 19;6:26227. (WB, Hu)
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