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Specific Keratinocyte Transglutaminase (Tgase-1) Colorimetric Microassay Kit : TG1-CovTest
Protein-glutamine gamma-glutamyltransferase K
Epidermal TGase
Gene name: TGM1, KTG
Epidermal TGase
Gene name: TGM1, KTG
As low as
$547.00
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00024794
A colorimetric microassay for quantification of Keratinocyte Transglutaminase (TG1) activity
| ID Covalab | opr0038 |
|---|---|
| Principe | The TG1-Covtest uses biotinylated K5 peptide (Biotin-pepK5) as the first substrate (amineacceptor/ acyl-donor) and spermine as second substrate (amine-donor/acyl-acceptor). In the presence of Tgase-1 (suspected to be contained in the samples), spermine is incorporated into the gamma carboxamide of the glutaminyl residue of the biotin-pepK5. The quantity of biotin incorporated is related to the Tgase-1 activity and is revealed by a peroxidase colorimetric reaction. |
| Applications | FA |
| Detection method | Colorimetric |
| Labelling | HRP |
| Activity cross reaction | Measurement of Transglutaminase-1 activity in biological samples with low or no interference with other TGs isoforms. |
| Species | All Sp. |
| Sample type | Biological fluids, cell and tissue lysates, recombinant Tgase-1 |
| Assay range | 0.003 - 0.15 mU/ml |
| Assay type | Colorimetric Microassay |
| Assay duration | 45 min |
| Storage instructions | DTT, Reaction Buffer B and SAv-HRP enzyme at -20°C. Other reagents at +4°C. |
| Target | Transglutaminase-1 (TGase-1) |
| Uniprot ID | P22735 |
| Gene ID | 7051 |
| Publication | Hitomi K, Kitamura M, Alea MP, Ceylan I, Thomas V, El Alaoui S. A specific colorimetric assay for measuring transglutaminase 1 and factor XIII activities. Anal Biochem. 2009 Nov 15:394(2):281-3. |
| Product type | Kits |
| Description | Microtiter strips with covalently bound spermine DTT EDTA (Negative Control) Reaction Buffer A (Biotin-pepK5/CaCl2) Reaction Buffer B Enzyme Tracer (SAv-HRP) Wash Buffer 10X Diluent Buffer 10X HRP Substrate Blocking Reagent |
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